Fig. 5

Ang-(1–7) improves histological results of spinal cord and bladder after SCI. a Microglial macrophage infiltration curves and time axis of histological and functional evaluation after SCI. b–c Double-staining for GFAP (green)/NF200 (red) and quantification of spinal cord sections in each group on the 28th day post-surgery. (scale bar: 500 μm, n = 4). Dashed line area shows cavity of spinal cord. d–e HE staining and quantification of bladder on the 28th day post-surgery. (scale bar: 100 μm, n = 5). f Continuous sections of the spinal cord by HE staining were obtained on the 28th day post-surgery, and the remaining neurons in the SCI group (f1, f2), SCI + Ang-(1–7) groups (f3, f4), and SCI + Ang-(1–7) + A779 groups (f5, f6) at the same distance from the epicenter were displayed. (scale bar: 100 μm and 40 μm, n = 3). Arrows indicate the remaining neurons. g Comparison of spinal cavity area at different sections. h Counting analysis of ventral neurons at rostral 2 mm and caudal 2 mm. (n = 5). ns nonsignificant, P > 0.05; *, #P < 0.05, **P < 0.01; ***, ###P < 0.001; and ####P < 0.0001. In a–e, h one-way ANOVA followed by Tukey’s Post Hoc test. In g, two-way ANOVA followed by Bonferroni’s post hoc tests