Fig. 3

Ang-(1–7) inhibits inflammation response by modulating the expression of inflammatory cytokines. a–b Quantitative RT-qPCR analysis of macrophages showing mRNA expression of IL-1β, TNF-α (a), IL-10, IL-4 (b) after treatment for 24 h. GAPDH: loading control. Data are expressed as fold change compared to the control group (n = 3). c–e Representative immunoblots and quantification showing protein expression of IL-1β (c, d) and TNF-α (c, e) in the macrophages after treatment for 24 h. β-actin: loading control. Data are expressed as fold change compared to the control group (n = 3). In a–e, data are presented as mean ± SD; ns nonsignificant, P > 0.05; *P < 0.05, **P < 0.01, and***P < 0.001. One-way ANOVA followed by Tukey's Post Hoc test