Fig. 1

Expression changes of MasR in macrophages in each group after 24 h. a Schematic diagram of macrophages extracted and induced from rat femur and tibia. b Double-staining for F4/80 (green)/ CD11b (red) in macrophages from each group (scale bar: 20 μm). c–d Double-staining for F4/80 (green)/ MasR (red) in macrophages from each group (scale bar: 50 μm, n = 5). e–f Representative Western blots and quantification of MasR and GAPDH expression in each group of macrophages treated for 6 h and 24 h. (n = 3). data are presented as mean ± SD; *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. In c-d, one-way ANOVA followed by Tukey’s Post Hoc test. In e–f, two-way ANOVA followed by Bonferroni’s post hoc tests