Fig. 3

Location and soma size of wFMNs post-BoNT/A WIM injection. a Schematic of the pathway of lFN at site of wFMNs projecting to WIM. b CTB-488-labelled neurons were all NeuN-positive, locating in the lFN 10 days after single CTB-488 or CTB-488-mixed BoNT/A injected into unilateral WIM. Scale bar, 500 μm (left one); 50 μm (right four). c Comparison of the soma size of effected neurons on lFN between the groups of single CTB-488 and CTB-488-mixed BoNT/A injection. n = 3 mice per group, unpaired two-tailed student’s t-test, t = 0.9621, df = 14, P = 0.3523. The neuron soma area in each group was normalized to the group of mice injected with single CTB-488. d Representative co-immunostaining image of ChAT and NueN. Scale bar, 500 μm. e All NeuN-positive cells expressing ChAT in the FN (n = 6 brain sections from 3 mice). n.s., non-significance; ACh, acetyl cholinergic pathway