Fig. 4
From: AMPK attenuates SHH subgroup medulloblastoma growth and metastasis by inhibiting NF-κB activation
AMPKα inhibits the proliferation but not migration of DAOY through GLI1. A Western blot detection of AMPKα, P- AMPKα and GLI1 families in DAOYs transfected with PRKAA1 siRNA or non-silencing control. B Western blot detection of AMPKα and GLI1 protein levels in DAOYs transfected with siPRKAA1 and siGLI1 alone or together. C Proliferation of DAOYs transfected with siRNAs were assessed by CCK-8. D Cell cycle of DAOYs transfected with PRKAA1 or GLI1 siRNA were detected by flow cytometry. E Quantification data of three independent experiments as in (D). F Transwell migration assay of DAOYs transfected with siRNAs and its quantification data (G) performed the average migration ± SEM from three independent experiments. *** P < 0.001