Fig. 2
From: Loss of TET2 impairs endothelial angiogenesis via downregulating STAT3 target genes
TET2 knockdown aggravates the hypoxia‐induced dysfunction of HUVECs. A, B Western blot of HUVECs transfected with Ctrl siRNA or TET2 siRNA under normoxia and hypoxia. C, D Dot blot assay of global 5-hmC using genomic DNA from HUVECs transfected with Ctrl siRNA or TET2 siRNA under normoxia and hypoxia. E, F EdU assay, G, H Scratch assay, I, J Tube formation assay, and K, L Apoptosis assay of HUVECs transfected with Ctrl siRNA or TET2 siRNA under normoxia and hypoxia. n = 3–4 per group. B Two-way ANOVA with Bonferroni post hoc test. D, F, H, J, L Unpaired Student’s t‐test. ns no significant; *, P < 0.05; **, P < 0.01; Nx normoxia, Hx hypoxia. E Scale bar, 50 μm. G, I Scale bar, 100 μm