Fig. 4

Dietary cholesterol and FGF19 in the mouse brain suppresses exploratory behavior. A 5-month-old male C56BL/6J mice (n = 16) underwent ICV osmotic minipump placement with aCSF (Alzet 0.5 µl /h) and were given 2-week recovery period. ICV pumps were then replaced with infusion of either FGF19 (15 ng/0.5 µl/h) or aCSF. Mice were placed into neurobehavioral open field testing chamber after being ad lib fed with a cholesterol-free diet (Teklad 2018) for 72 h. Using a cross-over design, mice underwent another ICV pump replacement and were given ICV infusion of either FGF19 or aCSF via osmotic minipump and placed on ad lib cholesterol-free diets 72 h prior to open field testing. One week later, mice were ad lib fed with the same diet supplemented with 1% cholesterol for 72 h and completed open field testing. B Open field testing results comparing mice receiving aCSF versus FGF19 infusion while on cholesterol-free diets. C Open field testing results comparing mice on cholesterol-deficient vs high cholesterol diet. *p ≤ 0.05. **p ≤ 0.01. ***p ≤ 0.001 by student t-test for (B) and 2-WAY ANOVA for (C)