Fig. 3

Dietary cholesterol results in increased FGF15 expression in the distal small intestine. A–C 10-week-old male C57BL/6 J mice were weight-matched (n = 8 per group) and divided into 2 groups with one group fed with cholesterol-deficient diet (Teklad 2018) and the other with the same diet supplemented with 1% cholesterol for 4 weeks. There were no differences in body weight, lean or fat mass. D–H Brain and small intestines were harvested at the same time under ad lib fed conditions. Semi-quantitative real-time RT-PCR was carried out on the hypothalamus and distal small intestine. I–J 10-week-old male C57BL/6 J mice were divided into several weight-matched groups, fed with cholesterol-deficient diets (Teklad 2018) for 48 h, fasted for 24 h (n = 12) and sacrificed, or fasted and refed with the same diet with or without cholesterol supplement (1%) for 12 h (n = 6 per diet) or 36 h (n = 6 per diet). Distal small intestinal tissue was harvested and mRNA expression for Fgf15 and Glp1 mRNA was examined. *p ≤ 0.05. **p ≤ 0.01. ***p ≤ 0.001 by 2-WAY ANOVA