Fig. 2

Locomotor activities are suppressed by dietary cholesterol, and this effect is partly governed by AgRP. A Experimental procedure. Briefly, a cohort of 7-month-old male Agrp^+/– (n = 6) and Agrp^–/– (n = 6) mice were transitioned from chow (Lab Diet 5058; cholesterol 200 ppm) to a cholesterol-free HFD diet (60% Kcal fat, 30.4% Kcal Carb, 9.6 kcal% protein, < 10 ppm cholesterol) and underwent metabolic measurements in CLAMS under thermoneutrality. The procedure was repeated 6 weeks later only this time the same mice were fed with the same diet containing cholesterol (280 ppm). B Food intake, C ambulatory activity, D vertical activity and E energy expenditure during the early night cycle (7PM-1AM) were compared between the two diets. F In a separate experiment, adult male Agrp^+/+ and Agrp^–/– mice (n = 7–9 per group; age 4–6 months old), maintained on a cholesterol-free chow diet (Teklad 2018), were placed in CLAMS at room temperature and ad lib fed with the same diet for first 2 days and then switched to the same diet supplemented with 1% cholesterol. G Food intake, H ambulatory activity, I vertical activity and J energy expenditure during the early night cycle (7PM-1AM) were compared. *p ≤ 0.05. **p ≤ 0.01. ***p ≤ 0.001 by two-way ANOVA with repeated measures