Fig. 1

Locomotor activities increase with food deprivation and are dependent on AgRP in a diet-specific manner. A–B Adult male Agrp^+/+ and Agrp^–/– mice (n = 6 per group; age 3 months) were placed in CLAMS at room temperature and ad lib fed with chow with cholesterol (Lab Diet 5058), and subsequently fasted for 24 h, as indicated by green line. In CLAMS while ad lib fed with cholesterol-deficient diets (Teklad 2018) at room temperature. Sum of ambulatory A or vertical activities B during the most active phase of the dark cycle (7PM to 1AM) were compared. C-D A separate group of adult male Agrp^+/+ and Agrp^–/– mice (n = 7–9 per group; age 5–6.5 months) were placed in CLAMS while ad lib fed with cholesterol-deficient diets (Teklad 2018) at room temperature. Sum of ambulatory C or vertical activities D during the most active phase of the dark cycle (7PM to 1AM) were compared. Data from second ad lib fed day in the CLAMS were compared with those from the equivalent time during the fasting period. Gray-shaded areas indicate dark cycles. Ns not significant. *p < 0.05. **p < 0.01. ***p < 0.001 by 2-WAY ANOVA with repeated measures