Fig. 5
From: SPOP inhibits BRAF-dependent tumorigenesis through promoting non-degradative ubiquitination of BRAF
The SPOP-BRAF interaction occurs in the cytoplasm. a, b Representative images of HeLa cells transfected with indicated plasmids, and then immunostained with Myc (SPOP), FLAG (BRAF), and DAPI. Scale bar, 10 μm. Enlargements of selected areas were shown. c Western blot of the indicated proteins in WCL and samples from co-IP with anti-FLAG antibody in 293 T cells transfected with the indicated plasmids. d 293 T cells were transfected with the indicated plasmids. The WCLs were prepared and the in vivo ubiquitination assays were performed. The polyubiquitinated forms of BRAF were detected by Western blot with anti-HA antibody