Fig. 6

Expression and subcellular localization of TPX2 during mouse oocyte meiotic maturation. A The protein content of TPX2 in oocytes at the GV, GVBD, MI, ATI, and MII stages was detected by immunoblotting. Protein loading was verified by the detection of β-actin. The molecular mass of target proteins is indicated on the right. B TPX2 was quantified for four independent repeats (normalized to β-actin, arbitrary units). Different lowercase letters above bars indicate statistical difference at p < 0.05 by ANOVA with the Tukey test. C Immunofluorescent localization of TPX2 in meiotic oocytes at various stages. D Spatial distribution of TPX2 and spindle microtubules in oocytes at different meiotic maturation stages. E Oocytes at the MI and MII stages were treated with taxol and then double-stained for TPX2 and α-tubulin. F Oocytes at the MI and MII stages were exposed to nocodazole and then co-stained for p-ADD1 and α-tubulin. Red, ADD1; green, α-tubulin; blue, DNA; Merge, overlapping of red, green, and blue. Bar, 20 μm