Fig. 6

NR4A1 attenuates malignant progression that induced by YTHDF2 in CC cells. A GEO database (GSE7410 and GSE63514) and GENT2 database of YTHDF2 mRNA in CC cohorts. The three lines inside the violin plots indicate the first quartile, median and third quartile. B Representative images of IHC staining for YTHDF2 protein from CC tissues and matched adjacent non-tumor tissues (n = 8, left) and quantitatively analyzed (right). C Transwell migration and invasion assays in CC cells expressing the specified plasmids. D, E The MTT (D) and colony formation assays (E) showed the cell proliferative ability with transfected the indicated plasmids in CC cells. F Western blot assay showed that the expression of N-cadherin, Vimentin and E- cadherin with transfected the indicated plasmids in CC cells. All experiments were performed at least 3 independent times, and data are presented as means ± SD except where otherwise specified. *P < 0.05, **P < 0.01, ***P < 0.001