Fig. 5

GBP treatment ameliorated the neurite and synaptic alteration, and the degenerated visual function following RI/R. A Western blotting showed the change of α2δ-1 expression in rat retinae following RI/R. B Bar graphs depicted the fold of optical density value (ODV) of α2δ-1 in each group (n = 6). C Western blotting showed the change in expression of α2δ-1 in rat retinae with FC treatment following RI/R. D Bar graphs depicted the fold of optical density value (ODV) of α2δ-1in each group (n = 6). E Western blotting showed the change in expression of α2δ-1 in rat retinae with TSP2 shRNA-AAV treatment following RI/R. F Bar graphs depicted the fold of optical density value (ODV) of α2δ-1in each group. (n = 6). G Diagram of GBP treatment before RI/R. H Co-immunoprecipitation depicted the binding of TSP2 and α2δ-1 in rat retinae with GBP treatment following RI/R. I Immunofluorescence images showed the morphological alteration of RGCs axons with GBP treatment following RI/R. J, K Bar graphs depicted the number of fascicles per view and the number of pNF + RGC axons per fascicle in each group (n = 7). L Western blotting showed the change in expression of synaptophysin (SYN), synapsin 1 and homer 1b/1c in rat retinae with GBP treatment following RI/R. M–O Bar graphs depicted the fold of optical density value (ODV) of SYN, synapsin 1 and homer 1b/1c in each group (n = 6). P Double-immunofluorescence images showed spatial location and origin of SYN in each group with GBP treatment following RI/R. Data in (B) were represented as mean ± SD; n.s.: no significance, (compared with the control group using one-way analysis of variance). Data in (D, F, J, K, M–O) were represented as mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.001, n.s.: no significance, (compared with the RI/R group using one-way analysis of variance). Bar = 50 μm in (I, P). The abbreviations are as defined in Fig. 1