Fig. 5
From: A synthetic pregnenolone analog promotes microtubule dynamics and neural development
P5 and its derivatives alter growth cone morphology. A The growth cone morphology of steroid-treated CGNs at DIV 2 immunostained with rhodamine-phalloidin for F-actin (red) and anti-β-tubulin antibody (green). Scale bar 5 µm. Quantification shows that B the total areas of the growth cone are reduced and C the percentage of transition zone area represented by the area doubly stained with IIIβ-tub and F-act are increased after treatment with P5 and its derivatives #2 and #43. D The scheme of measurement of the growth cone angle. E Quantification shows the reduced growth cone angle after neurosteroid treatment. All data were evaluated using one-way ANOVA, Dunnett’s post-hoc test