Fig. 4
From: Salidroside reduces neuropathology in Alzheimer’s disease models by targeting NRF2/SIRT3 pathway
SIRT3 KD inhibits SAL-mediated neurite and mitochondrial protection. A IF of Tuj1 (green) shows neurite morphology in Naïve and SIRT3KD SH-SY5Y cells with indicated treatment. Nuclei counterstained with DAPI. B Quantification of neurite length of A. C IF of TOM20 shows mitochondrial morphology in neurites of SIRT3KD SH-SY5Y cells with indicated treatment. Orange arrows, healthy mitochondria; white arrows, damaged mitochondria. Quantitation of images in C shows the length of mitochondrial segments (D) and mitochondrial density (E). F Fluorescence images show the intensity of aggregates (red) and monomers (green) of JC-1 staining in naïve and SIRT3KD cells with indicated treatment. G Quantification of JC-1 ratio of intensity in F. H Images show the fluorescence intensity of DCFH (ROS indicator) in cells with indicated treatment. I Quantitation of fluorescence intensity in H. Error bars indicate mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001; n.s., not significant; one-way ANOVA test. White scale bar, 50 μm; orange scale bar, 10 μm