Fig. 1

DNMT1 enhances breast cancer cells proliferation in vitro. A Analysis of DNMT1 expression in normal tissues and primary breast cancer tissues in TCGA database. B Analysis of DNMT1 expression in different subtypes of breast cancer tissues. C Detection of DNMT1 expression in breast cancer cell lines and MCF10A mammary epithelial cell line. D Assessment of MCF7-Vector cells and MCF7-DNMT1 cells proliferation abilities by CCK-8 assays. E, F CCK-8 analysis of MDA-MB-231 cells proliferation ability after knockdown DNMT1 or treated with DAC. G Western blots detected DNMT1, Cyclin-E2, Cyclin-D1, Cyclin-B1 and Cyclin-A2 expression in MCF7-Vector cells and MCF7-DNMT1 cells. H, I Detection of DNMT1, Cyclin-E2, Cyclin-D1, Cyclin-B1 and Cyclin-A2 expression by immune blots after depletion of DNMT1 or treated with DAC in MDA-MB-231 cells. Data are represented as mean ± SD of three or four independent experiments, and *p < 0.05, **p < 0.01, ***p < 0.001 (Student’s t test)