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Fig. 5 | Cell & Bioscience

Fig. 5

From: Vacuolin-1 enhances RA-induced differentiation of human myeloblastic leukemia cells: evidence for involvement of a CD11b/FAK/LYN/SLP-76 axis subject to endosomal regulation that drives late differentiation steps

Fig. 5

Deletion of CD11b or Numb attenuates RA-induced HL-60 cell differentiation. A The sg-NC (non-specific guide control), sg-CD11b (CD11b targeted CRISPR KO) and sg-Numb (Numb targeted CRISPR KO) stably transfected HL-60 cells were collected for WB analysis of CD11b and Numb. B The sg-NC, sg-CD11b and sg-Numb HL-60 cells were cultured for 72 h with or without (Control) 1 µM RA as indicated and membrane CD11b was analyzed using flow cytometry. C–E The sg-NC, sg-CD11b and sg-Numb HL-60 cells were cultured for 72 h with or without 1 µM RA as indicated, and membrane CD38 (C), inducible ROS induced by TPA D and G1/G0 cell cycle arrest E were analyzed as indicated by flow cytometry. DMSO is the carrier control (the negative) for TPA (the positive) used to induce oxidative metabolism (Reactive Oxygen Species, ROS)

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Cell & Bioscience

ISSN: 2045-3701

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