Fig. 6

Structure and function of 3D U-iKOs. A Immunofluorescence analysis of fluorescence-labeled dextran uptake assay in 3D U-iKOs for 10 kDa, 70 kDa, and 2000 kDa of dextran. Scale bars, 50 μm. B TEM of 3D U-iKOs (day 24). Podocyte (p) with characteristic large nuclei and primary processes (pp) and secondary processes (sp) in the upper panel. A putative proximal tubule cells with enriched mitochondria (mit) and a lumen filled with closely packed microvilli and the brush border (bb) in the middle panel. A putative distal tubule with relatively sparse short microvilli (m) and tight junctions (tj) in the bottom panel. Scale bars, 2 μm. C H and E staining of implanted kidney organoids in the CAM for 6 days. Magnified views of glomerular (G) and tubular (T) cells are shown. Scale bars, 100 μm. D Confocal microscopy images of implanted organoids in C stained for parietal epithelial cells maker (PAX8), bowman’s capsule basement membrane (LAM), and tubular markers (LTL, CDH1, and PAX8). Scale bars, 50 μm