Fig. 3

Efficient maintained U-iIMPLCs retain nephrogenic potential. A Cell morphology of U-iIMPLCs subcultured at different passages. The same strain of U-iIMPLCs 1# with different passages P0, P1, P5, P9, P17, and P21 are presented. Scale bars, 250 μm. B Growth curve of U-iIMPLCs for different strains of U-iIMPLCs 2#, 6#, and 8#. C Correlation analysis showing gene expression profiles of hUCs, hPSCs, and U-iIMPLCs 1# maintained at different passages. D Gene expression analysis for hUCs, intermediate mesoderm, paraxial mesoderm, Lateral plate mesoderm, pluripotent, ectoderm, and endoderm markers of the cells shown in C. E Gene expression analysis for intermediate mesoderm progenitor markers for different strains with different generations. The relative expression of each transcript to GAPDH expression is presented as the mean ± SEM. F Representative stereo microscope images showing 2D kidney organoids differentiated from the same strain of 8# U-iIMPLCs at different passages in a single well of a 24-well plate. Scale bars, 500 μm. G The number of 2D U-iKOs in F. H Representative stereo microscope images showing 2D kidney organoids differentiated from different strains at passage 10. Scale bars, 500 μm. I The number of 2D kidney organoids is shown in H