Fig. 1
From: Krüppel-like factor 7 deficiency causes autistic-like behavior in mice via regulating Clock gene
Regulation of circadian rhythm genes by klf7. A qPCR analysis of diurnal rhythm expression of klf7 in N2A cells. Peak presents at CT16 and nadir presents at CT0 (n = 4 independent experiments). B qPCR analysis of the expression of circadian rhythm genes induced by klf7 knockdown in N2A cells. Shown is fold change induced by klf7 knockdown (n = 4 independent experiments). C qPCR analysis of the expression of circadian rhythm genes induced by klf7 overexpression in N2A cells. Shown is fold change induced by klf7 (n = 4 independent experiments). D RNA-seq analysis of the expression of circadian rhythm genes in klf7 knockdown human brain organoid model compared with control group. Shown is log2(fold change) induced by klf7 knockdown (n = 2 independent experiments). The data are presented as the mean ± SEM. Statistical analysis (P* < 0.05, P** < 0.01 and P*** < 0.001) between control group and klf7 knockdown group in (B) were performed by unpaired t test. Statistical analysis (P* < 0.05, P** < 0.01, P*** < 0.001, P**** < 0.0001 and ns: no significance) between control group and klf7 group in (C) were performed by unpaired t test