Fig. 4

Yap1 deletion promoted LPS-induced accumulation of ROS and lipid peroxidation in vitro. Primary hepatocytes from YAP1^fl/fl and YAP1^fl/fl Alb-Cre mice and LO2 cells were treated with LPS (1 µg/mL) for 24 h. Representative images of fluorescence probes (DCFH-DA) for intracellular ROS production of primary hepatocytes (A) and LO2 cells (G) and the quantification of ROS fluorescence intensity respectively, n = 3 (B, H). Cell viability was evaluated by the cell counting kit-8 of primary hepatocytes (C) and LO2 cells (K) respectively, n = 3. The contents of MDA (D, L), GSH (E, M) and Fe²⁺ (F, N) of primary hepatocytes and LO2 cells were determined using the indicated kits, n = 3. I Lipid ROS generation was analyzed by using BODIPY 581/591 and determined by flow cytometry in LO2 cells treated with LPS and J quantification of Lipid ROS, n = 3. Data are expressed as mean ± SD of three replicates, *p < 0.05, **p < 0.01, ***p < 0.001