Fig. 1
From: Hif-2α regulates lipid metabolism in alcoholic fatty liver disease through mitophagy
Upregulation of Hif-2α in vivo and in vitro. C57 male mice were fed an EtOH liquid diet or the control diet for 16 days. AML-12 cells were treated with 100 mM EtOH for 48 h. A, B Representative Oil red o staining images and IHC-Hif-2α of liver tissue samples from EtOH-fed mice (scale bar = 100 μm or 20 μm) (n = 6). C, D qRT-PCR and western blotting analysis of Hif-2α and E, F CPT-1α and MCAD expression in EtOH-fed mice (n = 6) and EtOH-treated AML-12 cells (n = 3). G Representative Oil red o staining images of EtOH-treated AML-12 cells (scale bar = 100 μm) (n = 3). Data are presented as the mean ± S.D. *P < 0.05, **P < 0.01