Fig. 2

Oxy186 and Oxy210 inhibit A549 cell proliferation through non-canonical GLI and WNT/β-catenin pathways. A. Basal 8xGliBS-Luc reporter activity in A549 cells treated with Oxy186, Oxy210, the GLI inhibitor GANT61, or the SMO inhibitor cyclopamine. Data from a representative experiment are reported as the mean of triplicate measurements ± SD (*p < 0.05 vs. DMSO control). B. GLI inhibitor GANT61 and WNT pathway inhibitor FH535, but not TGF-β type I receptor inhibitor SB431542, inhibit A549 cell proliferation. Data from a representative experiment are reported as the mean of quadruplicate measurements ± SD (**p < 0.02; ***p < 0.001 vs. DMSO control). C. Oxy186 (10 µM) and Oxy210 (10 µM) further inhibited A549 cell growth in the presence of GLI inhibitor GANT61 or WNT pathway inhibitor FH535. Data from a representative experiment are reported as the mean of quadruplicate measurements ± SD (***p < 0.001)