Fig. 5

ROS-IRE1α-JNK pathways are related to autophagic cell death induced by CUDC-907. a Western blot analysis of p-IRE1α/IRE1α, p-JNK/JNK, CHOP, and cleaved caspase 12 in CUDC-907 (30 nM)-treated KYSE-450 and KYSE-510 cells in a time-dependent manner. b Ratio of p-IRE1α/GAPDH and p-JNK/GAPDH in CUDC-907-treated ESCC cells determined using Image J. c, d Western blot analysis of p-IRE1α/IRE1α, p-JNK/JNK, CHOP, and LC3B-I/lC3-II. Cell viability and LDH release of CUDC-907 (30 nM, 48 h)-treated KYSE-450 and KYSE-510 cells with siIRE1α or negative control treatment for 48 h. e Western blot analysis of p-JNK/JNK, Beclin1, and LC3B-I/lC3-II in CUDC-907 (30 nM, 48 h)-treated ESCC cells (KYSE-450 and KYSE-510) with SP600125 (10 μM) or not for 48 h. f Western blot analysis of p-IRE1α/IRE1α, p-JNK/JNK in CUDC-907 (30 nM, 48 h)-treated KYSE-450 and KYSE-510 cells, pretreated with NAC (5 mM) or no treatment for 6 h. Error bars are ± SD. *P < 0.05; **P < 0.01; ***P < 0.001