Fig. 2

Inhibition of SENP6 promoted microglial polarization towards an anti-inflammatory phenotype after OGD/R treatment. A, B Primary cultured microglia were infected with adenovirus expressing wild-type SENP6 or shRNA against SENP6 following OGD/R treatment. The mRNA levels of OGD/R-induced proinflammatory (A) and anti-inflammatory (B) phenotype marker genes were detected by RT–qPCR. C Immunoblots showing the protein expression of iNOS, CD16/32, Arginase-1 and CD206 in primary microglia infected with Ad-SENP6 or Ad-sh. SENP6. D–G Quantification analysis of the protein levels in C. H–M The supernatant of primary microglia was collected, and cytokine levels were detected by ELISA. Data are presented as the mean ± S.E.M. from at least three dependent experiments and analysed by one-way ANOVA followed by Tukey’s post-hoc test. ns for P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001