Fig. 2

CRLM1 inhibits CRC cell apoptosis and promotes metastasis in vitro and in vivo. A, B The relative expression of CRLM1 was detected in HCT116 and SW620 cell lines after transfection with overexpressed CRLM1 plasmid (a) or si- CRLM1 (b) by qRT-PCR. C, D The apoptosis rate was analyzed by flow cytometry after downregulation of CRLM1 (C) and the quantification data (D). E–H The migratory ability of HCT116 (E) and SW620 (F) cell lines were detected by wound healing assays after overexpression or knockdown of CRLM1 (magnification, ×200, scale bar, 100 μm) and the quantification data (G, H). I–K Transwell assay was used to measure CRC cells invasion abilities after overexpression or knockdown of CRLM1 (magnification, ×200, scale bar, 100 μm) (I) and the quantification data (J, K). L–O Liver metastasis were taken from Balb/c nude mice injected with CRLM1-OE or sh-CRLM1 SW620 cells into the distal tip of the spleens for 4 weeks (n = 4 for each group). The images of liver metastasis (L, N) and the quantification data (M, O). Data are shown as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001