Fig. 2

SaCas9 and KKH SaCas9 are efficient and robust tools for genome editing in X. tropicalis. a SaCas9 induced efficient targeted gene disruption in X. tropicalis embryos. b KKH SaCas9 induced efficient targeted gene disruption in X. tropicalis embryos. c, d Deep-sequencing data show some of the mutations induced by SaCas9 and KKH SaCas9. For all the panels, the wild-type sequence is shown at the top with the target site highlighted in yellow and the PAM sequence in blue text. Red dashes indicate deletions and lowercase letters in red indicate insertions or mutations. The numbers in parentheses show the percentage of this sequence in the total sequence reads. For target site designation, the gene name is in the middle, the prefixes Sa- and KKH- represent SaCas9 and KKH SaCas9, respectively. The suffixes -T1 and -T2 represent the first and the second target site designed in a given gene, respectively