Fig. 8

Inh. XII, but not KT5720, prevent canonical signalling of NF-kB from being activated after 2 h of high glucose challenge. rMC1 cells were pre-treated with 10 µM KT5720, Inh. XII or DMSO for 2 h. Thereafter, 25 mmol/L glucose or the same volume of 5 mmol/L glucose was administered. Whole cell lysates were prepared at 2 and 3 h after glucose delivery and analysed by Wb. Filters were probed with the anti-IkBα(Ser32) antibody. Remarkably, epoxomicin was not enrolled due to increase of pIkBα(Ser32) at these time-points as indicated in Additional file 1: Fig. S4. Total proteins stained by Ponceau S were used as loading control. A representative experiment of three independent replicates is shown. Histogram represents the relative levels of pIkBα(Ser32) protein in the different experimental conditions. One-way ANOVA followed by Tukey’s post-hoc student test: **p < 0.01, *** < p.0.001