Fig. 3

DNMT1 inhibition attenuated HK-2 cell and human podocyte injury in vitro. A and B The expression of HBx and DNMT1 in HBx-HK-2 cells and HBx-podocytes with or without DNMT1 knockdown was evaluated by western blotting. C and D The localization and expression of HBx and DNMT1 in HBx-HK-2 cells and HBx-podocytes were determined by immunofluorescence. Scale bar: 10 μm. E and F ELISA was conducted to measure concentrations of IL-6 and TNF-α in the culture supernatant of HBx-HK-2 cells and HBx-podocytes with or without DNMT1 knockdown. G and H Western blotting was conducted to measure the expression of E-cadherin, ZO1, Vimentin, Fibronectin, Podocin, and Nephrin in HBx-HK-2 cells and HBx-podocytes with or without DNMT1 knockdown. Results are expressed as mean ± SD of 3 separate assays. *P < 0.05; **P < 0.01