Fig.1

The chromatin accessibility dynamics during PST. A Schematic for JUN induced pluripotent to somatic transition (PST) and time course sample collection. ESCs containing JUN^TetON and switched into medium plus/minus doxycycline(Dox) and then harvested for ATAC-seq at the time points indicated in the upper row. Time points: 0 h, 4 h, 8 h, 12 h. B Heatmap showing the chromatin accessibility dynamic changes during PST. Loci of chromatin were arranged into groups depending upon the day of PST they changed from closed to open (CO) or open to closed (OC) or when they were permanently open (PO). PO was subdivided into those loci that declined (but remained open) as PO-down(POD), those that increased as PO-up(POU), and those that were unchanged (PON). CO and OC were subdivided CO1-3, OC1-3 respectively. C The number of peaks defined in each of the OC/CO and PON/POU/POD categories. D Motif analysis for CO1 and CO2 during JUN based PST. Table lists for the top10 most enrichment motifs. Peaks number = 34,661, 7949, respectively. E Heatmap of RNA-seq data during JUN based PST. Three groups have been divided, gruop1 and group2 indicate gene upregulated, and group3 indicates gene downregulated. F Correlation between ATAC-seq and RNA-seq. Each group of ATAC-seq binding sites are mapped to the TSS–/ + 10 kb, these gene are overlap to RNA-seq. Then we calculate the percentage of three group RNA-seq in each ATAC group which got by overlap ATAC-seq and RNA-seq, indicated by pie chart. G Calculating the percentage of every ATAC group in each RNA-seq group which got by overlap ATAC-seq and RNA-seq, indicated by pie chart. H Selected genomic views of the ATAC-seq data were shown for the indicated PON, POU, POD, CO, OC groups. Loci of indicated groups are marked with gray boxes. The RNA-seq expression values for the respective genes are shown below. RNA-seq expression units are in TPM (Transcripts Per Million reads), the following is the same