Fig. 6

Rbpjl alleviates AP by downregulating Arid5a and blocking the IL-6/STAT3 axis in vivo. A Rbpjl and Arid5a protein expression in pancreatic tissues of mice treated with oe-Rbpjl or combined with oe-Arid5a determined by Western blot assay. B The intranuclear STAT3 protein expression, total protein expression of STAT3 as well as STAT3 phosphorylation level in pancreatic tissues of mice treated with oe-Rbpjl or combined with oe-Arid5a were measured by Western blot assay. C HE staining was used to observe the degree of pancreatic injury, and the degree of pancreatic injury was graded and evaluated in mice treated with oe-Rbpjl or combined with oe-Arid5a. D The weight ratio of pancreatic tissues to total body in mice treated with oe-Rbpjl or combined with oe-Arid5a was detected. E TUNEL staining was used to detect the apoptosis rate in pancreatic tissues of mice treated with oe-Rbpjl or combined with oe-Arid5a. F The expression of pro-inflammatory factors in the serum of mice treated with oe-Rbpjl or combined with oe-Arid5a was detected by ELISA. The mouse experiments were performed 14 days after AP modeling, with n = 8 for mice in each group