Fig. 4

Arid5a activates the IL-6/STAT3 axis in pancreatic acinar cells. A The interaction network of Arid5a in the STRING website. B The interaction network of IL-6 in the STRING website. C Detection of intranuclear protein expression of STAT3 and STAT3 phosphorylation level in the pancreatic tissues of AP mice and LPS-induced MPC-83 cells (for 6 h) by Western blot assay (* p < 0.05 vs. sham-operated mice. ^# p < 0.05 vs. control MPC-83 cells). D The expression of intranuclear STAT3 protein, Arid5a, total protein expression of STAT3 as well as STAT3 phosphorylation level in the LPS-induced MPC-83 cells (for 6 h) treated with sh-Arid5a was measured by Western blot assay (* p < 0.05 vs. LPS-induced MPC-83 cells treated with sh-NC). E The expression of pro-inflammatory factor IL-6 in LPS-induced MPC-83 cells (for 6 h) treated with sh-Arid5a was detected by RT-qPCR (* p < 0.05 vs. LPS-induced MPC-83 cells treated with sh-NC). The mouse experiments were performed 14 days after AP modeling, with n = 8 for mice in each group. The cell experiment was conducted three times independently