Fig. 2

FANCC downregulation of inflammation in microglia is p38/NLRP3 dependent. A Western blotting performed for the proteins including p-p38, p38, NLRP3, pro-Caspase-1, cleaved-Caspase-1, pro-IL-1β and cleaved-IL-1β in LPS-activated microglia pretreated with BIRB 796 and glyburide after transfection with OE-FANCC; n = 3. β-actin was used as the control. B Bar graph showing the ratio analysis of p-p38/p38. C Densitometric analysis of NLRP3 expression. D Densitometric analysis of pro-Caspase-1expression. E Densitometric analysis of cleaved -Caspase-1expression. F Densitometric analysis of pro-IL-1β expression. G Densitometric analysis of cleaved -IL-1β expression. H, I ELISAs performed for the IL-1β and IL-18 in culture medium obtained at 24 h in LPS-activated microglia pretreated with BIRB 796 and glyburide after transfection of OE-FANCC (n = 5). J Representative flow cytometry performed for the distinction of iNOS⁺&F4/80⁺ microglia pretreated with BIRB 796 or glyburide and transfected OE-FANCC post 24 h LPS inducement. K Bar graph showing the quantitative analysis of the percent; n = 4. The error bars represent the SD. *p < 0.05 vs. NC-PBS group, #p < 0.05 vs. NC-LPS group, &p vs. OE-LPS group by one-way ANOVA followed by Tukey’s post hoc analysis (*p < 0.05, **p < 0.01, and ***p < 0.001)