Fig. 6
HDAC3 deficiency in macrophages aggravates pseudomonas aeruginosa induced acute lung injury due to impaired inflammatory response. A, B ELISA analysis of IL-6 and TNFα in BALF of LysmCre and LysmCreHdac3f/f mice uninstilled or intratracheally instilled with LPS (5 mg/kg) (12 h). C Survival curve of 8-week-olds LysmCre and LysmCreHdac3f/f mice intratracheally received with pseudomonas aeruginosa [Colony forming unit (CFU) = 2 × 107]. PA, Pseudomonas aeruginosa. D Temperature of LysmCre and LysmCreHdac3f/f mice intratracheally received with pseudomonas aeruginosa (CFU = 2 × 107) monitored for 36 h. E CFU of pseudomonas aeruginosa in BALF of LysmCre and LysmCreHdac3f/f mice uninstilled or intratracheally instilled with pseudomonas aeruginosa (CFU = 2 × 107) for 36 h was calculated. F, G H&E staining of LysmCre and LysmCreHdac3f/f mice intratracheal unreceived or received with pseudomonas aeruginosa (CFU = 2 × 107) for 36 h and the lung injury score was calculated. Scale bar: 50 μm. H Pulmonary hemorrhage of LysmCre and LysmCreHdac3f/f mice received with pseudomonas aeruginosa (CFU = 2 × 107) for 36 h was judged by the color of BALF. I ELISA analysis of HMGB1 in the BALF of LysmCre and LysmCreHdac3f/f mice uninstilled or intratracheally instilled with pseudomonas aeruginosa (CFU = 2 × 107) for 36 h. J Schematic diagram of how HDAC3 regulates RIP1 mediated inflammatory response of macrophages. Data are showed as mean ± SEM; n = 4–9 mice per group; *P < 0.05 and **P < 0.01