Table 1 RT-qPCR study of altered mRNA processing in different tissues of E16 mice
From: Hepatitis, testicular degeneration, and ataxia in DIDO3-deficient mice with altered mRNA processing
Organ | Target | Dido1 genotype | |||||
|---|---|---|---|---|---|---|---|
WT (n = 3) | E16 (n = 3) | ||||||
Liver | Gas2l1 | 2.1 | 5.6 | 2.7 | > 9.4 | 2.2 | 1.1 |
Osbpl2 | 6.0 | 5.5 | 5.6 | − 1.5 | 0.2 | 0.1 | |
Ndufs7 | 15.6 | 17.1 | 15.3 | > 12.4 | 11.8 | 12.0 | |
Wrap73 | 7.2 | 8.9 | 9.1 | − 0.2 | 1.2 | 1.7 | |
Testis | Gas2l1 | 1.5 | > 6.4 | > 6.1 | 0.9 | 1.5 | 2.1 |
Osbpl2 | 2.3 | 5.2 | 4.3 | 0.6 | 1.6 | 2.6 | |
Ndufs7 | > 13 | > 16.8 | > 18.1 | 11.5 | 9.7 | 17.0 | |
Wrap73 | 6.2 | 7.7 | 7.9 | 2.9 | 3.0 | 3.5 | |
Brain | Gas2l1 | 6.7 | > 6.8 | > 5.5 | 1.1 | 1.2 | 6.7 |
Osbpl2 | 4.4 | 7.0 | 6.5 | − 0.7 | 1.5 | 0.9 | |
Ndufs7 | > 13.8 | > 18.1 | > 18.3 | 10.7 | 11.9 | 17.5 | |
Wrap73 | 10.1 | > 13 | > 12.9 | 3.6 | 3.4 | 5.7 | |
Heart | Pick1 | > 8.6 | > 7.0 | > 2.6 | 5.0 | 6.2 | 4.0 |
Gas2l1 | 3.3 | > 6.3 | > 6 | − 1.3 | 1.5 | 2.5 | |
Osbpl2 | 5.2 | 8.2 | > 10.6 | − 0.5 | 0.9 | 3.9 | |
Ndufs7 | 15.8 | > 20.9 | > 20.3 | 7.5 | 11.2 | > 19.5 | |
Wrap73 | 8.5 | > 7.9 | > 7.7 | 3.6 | 3.7 | 2.3 | |
Lung | Pick1 | > 6.4 | > 4.1 | > 3.8 | 3.6 | 5.4 | 5.0 |
Gas2l1 | 3.6 | > 7.1 | > 6.7 | − 0.9 | 1.0 | − 0.3 | |
Osbpl2 | 4.4 | 6.3 | > 3.6 | − 1.0 | 2.3 | ND | |
Ndufs7 | 13.8 | > 17.4 | > 14.8 | 8.7 | 10.3 | > 10 | |
Wrap73 | 8.1 | 7.3 | > 10.5 | 1.9 | 2.1 | 3.9 | |
Duodenum | Pick1 | > 6.0 | 8.3 | ND | 3.0 | ND | 1.1 |
Gas2l1 | 6.2 | 5.6 | > 3.9 | 7.0 | − 0.4 | 1.7 | |
Osbpl2 | 2 | 4.4 | 2.0 | − 1.1 | − 1.2 | − 0.6 | |
Ndufs7 | > 11.8 | > 18.6 | > 16.2 | > 9.7 | 9.1 | 11.3 | |
Wrap73 | 8.2 | 6.4 | > 10.0 | 4.0 | 1.5 | 1.1 | |
Average ΔCtA-N (lower limit) | > 8.6 | > 4.2 | |||||