Fig. 8

Tlr4 is expressed in RGC axons, astrocytes, and microglia in the mouse ONH. Tlr4 mRNA shown as white puncta in all images by RNAScope in situ hybridization in combination with immunofluorescent labeling of axons, astrocytes, and microglia. A Tlr4 mRNA is detected in RGC axons (green), B in ONH astrocytes (blue) and C in ONH microglia (magenta). A’ and A” are magnified fragments of A; B’ and B” are magnified fragments of B; C’ and C” are magnified fragments of C. Yellow arrows point to localization of Tlr4 mRNA in cells positive for specific markers. D Bar graph showing percent of Iba1 positive cells with detected Tlr4 mRNA in C57BL/6J control and B6.EDA^+/+ mice. Significance determined by Student’s t-test. Scale = 50 µm. n = 3 mice/strain