Fig. 3

B6.EDA^+/+ mice have increased amorphous ECM and changes to basement membrane material along the inner wall of SC. A, B Fragment of the stitched image of the entire length of SC (A) and a representative example of how the percent length of BMM along the inner wall of SC was measured (B). The inner wall of SC is marked with a green line and BMM marked with red a line. C Percent of BMM covering the length of the SC in C57BL/6J and B6.EDA^+/+ mice. D, E Large portions of the inner SC endothelium is covered by amorphous ECM material (red arrows) in B6.EDA^+/+ mice, which may conceal the BMM (yellow arrows), and makes it more difficult to detect of BMM in these regions. F Length of SC that is unquantifiable for BMM due to amorphous material under the endothelium in C57BL/6J and B6.EDA^+/+ mice. n = 3 mice/strain, two planes of stitched sections along the entire length of SC analyzed per mouse, scale bar = 1 µm.