Fig. 1
From: LRP4 is required for the olfactory association task in the piriform cortex
Highly expression of Lrp4 in piriform cortex. A X-gal staining of Lrp4+/Lac mice brain slice. PIR, piriform cortex; CTX, cerebral cortex; HIP, hippocampus; TH, thalamus; Lrp4+/Lac mice, n = 5. B Lrp4 expression in wild-type adult mice brain was confirmed by Western blotting (Wild type mice, n = 6). C Quantification of LRP4 protein level relative to α-tubulin from Western blotting. LRP4 relative level in the HIP was normalized to a value of 1. D mRNA expression of Lrp4 in piriform cortex in postnatal wild-type mice. Lrp4 expression values were calculated relative to Gapdh by using the 2−ΔCT methods, and the P18 group was set to a value of 1. (Wild type mice per group, n ≥ 5; RNA pool was made for qPCR in each group; Values were means ± SEM, one-way ANOVA with multiple comparisons, compared other group with P18 group. n.s. no significant, * P < 0.05, *** P < 0.001)