Fig. 6
From: Rho family GTPase 1 (RND1), a novel regulator of p53, enhances ferroptosis in glioblastoma
RND1 induced the ferroptosis via the p53-SLC7A11 signaling in GBM. A Knockdown of RND1 significantly decreased the p53-driven luciferase activity in U87 cells by dual luciferase reporter assays. B Knockdown of RND1 increased the mRNA level of SLC7A11. C HA-p53 reversed the effects of RND1 knocked down on p53 and SLC7A11 protein levels in U87 cells. D PFT-α (an inhibitor of p53) reversed the effects of RND1 overexpression on p53 and SLC7A11 protein levels in U87 cells. E According to the dual luciferase reporter assays, PFT-α reversed the activity of p53 signaling when RND1 was overexpressed. F–H PFT-α reversed the effects of RND1 overexpression on the ROS, GSH level and MDA level in U87 cells. *P < 0.05; **P < 0.01; ***P < 0.001; ns, no significance