Fig. 5

Gain of function of VHL mutation promotes epithelial-mesenchymal transition (EMT). A, B GSEA-determined EMT pathways with VHL S65P (A) or S65W-influenced gene signature (B). Genes are ranked by their transcriptional levels in S65P (A) or S65W (B) expressed 786-O cells. Right panels of A or B indicate HIF2α ChIP-seq signals of 5 kb around TSS of EMT pathway genes influenced by VHL S65P (A) or VHL S65W (B). C Western blot of the EMT marker VIMENTIN protein levels across 786-O cell lines expressing empty vector (EV), VHL WT or mutants. D Left panel: representative images of invasion assays for control 786-O cells or the 786-O cell lines with VHL or VHL mutant expression. Right panel: quantitation of relative invasion for 786-O cells. E Left panel: representative images of migration assays for control 786-O cells or the 786-O cell lines expressing VHL or VHL mutants. Right panel: quantitation of relative migration for 786-O cells. In D,E, error bars s.d. n = 3 technical replicates. P values were assessed via the two-tailed Student’s t test