Fig. 2
From: Analysis of CCR2 splice variant expression patterns and functional properties
Analysis of receptor interactions with β-arrestins or endosomal markers using the NanoBiT assay. C-terminal LgBiT forms of CCR2A (A) or CCR2B (B) were co-expressed with β-arrestin 1-SmBiT or β-arrestin 2-SmBiT in HEK293 cells. The cells were incubated with the luciferase substrate and treated with MCP-1. Luciferase activity was measured with a luminometer for 70 min. The graphs are representatives of more than three independent experiments. C The amino acid sequences of the third intracellular loop and C-terminal regions of the variants. Blue colors indicate Ser/Thr residues. Red colors indicate the amino acid sequences that differ between the variants. D HEK293 cells expressing SmBiT-Clathrin and b-arrestin1-LgBiT with each of CCR2 variants were treated with 100 ng/ml MCP-1 and the luminescence change was measured. E HEK293 cells co-expressing the C-terminal LgBiT forms of the CCR2 variants and SmBiT-FYVE were treated with 100 ng/ml MCP-1 and the luminescence change was measured