Fig. 5

KMT2D cooperates with MEF2A to initiate the transcription of CTNNB1. A Correlation analysis of expression of KMT2D and β-catenin generated from GEPIA. B Immunoblotting and quantification analysis of β-catenin and downstream of canonical Wnt/β-catenin pathway in SCC4 and SCC25 cells transfected with Sh-CON and Sh-KMT2D. C Quantification analysis of immunoblotting of β-catenin and downstream of canonical Wnt/β-catenin pathway in SCC4 and SCC25 cells transfected with Sh-CON and Sh-KMT2D. D Immunoblotting of β-catenin and downstream of canonical Wnt/β-catenin pathway in SCC4 and SCC25 cells transfected with Sh-KMT2D treated with DMSO and Wnt/β-catenin agonist 1. E Co-IP experiments for KMT2D and MEF2A in SCC25 cells. F Relative transcriptional activity of CTNNB1 in SCC4 and SCC25 cells transfected MEF2A overexpression and control. G Immunoblotting of MEF2A, β-catenin, and CyclinD1 in SCC4 and SCC25 cells with MEF2A overexpression and control. H Relative transcriptional activity of CTNNB1 in SCC4 and SCC25 cells transfected with Sh-KMT2D and control. Data are presented as means ± SD. Results are representative of at least three independent experiments. GEPIA Gene Expression Profiling Interactive Analysis, SD standard deviation