Fig. 2

KMT2D contributes to the self-renewal ability of primary OSCC cells. A Representative images showed the immunofluorescence staining of KMT2D in patient-derived organoids generated from OSCC and paracancerous specimens, as well as their parental tissues. (scale bars = 50 μm). B Representative images indicated the organoid reconstitution assay (i.e. organoid morphology and clone size) conducted for OSCC cells transfected with Sh-CON and Sh-KMT2D. C Quantificational analysis showed the organoid reconstitution assay (i.e. organoid forming efficiency) conducted for OSCC cells transfected with Sh-KMT2D and Sh-CON. Student's t-test. D Representative images showed the immunofluorescence staining of β-catenin and CD133 in patient-derived OSCC organoids transfected with Sh-CON and Sh-KMT2D. (scale bars = 50 μm) Results are representative of at least three independent experiments(*p < 0.05,**p < 0.01, and ***p < 0.001). Data are presented as means ± SD. OSCC oral squamous cell carcinoma, SD standard deviation