Fig. 4
LYPLAL1-AS1 overexpression attenuates hADSC senescence. A Representative images of hADSCs transfected with lentivirus expressing GFP and control (Lenti-NC) or LYPLAL1-AS1 (Lenti-LYPLAL1-AS1). B qRT-PCR of overexpression efficiency of LYPLAL1-AS1 in hADSCs transfected with control or LYPLAL1-AS1 overexpression lentivirus. C SA-β-Gal staining in control and LYPLAL1-AS1 overexpression P10 hADSCs. D qRT-PCR of P16, P21, and LMNB1 in control and LYPLAL1-AS1 overexpression P10 hADSCs. E Western blot of P16, P21, and LMNB1 in control and LYPLAL1-AS1 overexpression P10 hADSCs. F Western blot of P16, P21, and LMNB1 in control and LYPLAL1-AS1 overexpression H2O2-treated hADSCs. G SA-β-Gal staining in control and LYPLAL1-AS1 overexpression H2O2-treated hADSCs. qPCR data were normalized to GAPDH, n = 3. Data are shown as mean ± SD from three independent experiments; *p < 0.05, **p < 0.01, ****p < 0.0001; scale bars: 200 µm