Fig. 1
Characterization of hADSC senescence. A SA-β-Gal staining of hADSCs during replicative senescence at different passage(P): P3, P9, P12, and P15. B qRT-PCR of senescence markers P16, P21, and LMNB1 in hADSCs during replicative senescence. C Western blot of P16, P21, and LMNB1 in hADSCs during replicative senescence. D SA-β-Gal staining of H2O2-treated hADSCs. E qRT-PCR of P16, P21, and LMNB1 in H2O2-treated hADSCs. F Western blot of P16, P21, and LMNB1 in H2O2-treated hADSCs. qPCR data were normalized to GAPDH, n = 3. Data are shown as mean ± SD from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; scale bars: 200 µm