Fig. 9

Adoptive transfer of tolDCs restores the balance of Th17/Treg cells in experimental colitis mice. On day 3 after TNBS administration, the mice from each group were euthanized to collect cells from the spleen, and MLNs were collected and stained with anti-CD4, anti-CD25, intracellularly stained with anti-IL-17A and anti-Foxp3. The percentages of Th17 and Treg cells were measured by flow cytometry. Data are shown in the form of flow cytometry chart or expressed as the Mean ± SD (n = 5 mice per group). A, C The representative plots of Th17 and Treg cells in spleen. B, D Quantitative analysis of the percentages of Th17 and Treg cells in spleen. G The representative plots of Th17 and Treg cells in MLNs. F, H Quantitative analysis of the percentages of Th17 and Treg cells in MLNs. MLNs, mesenteric lymph nodes. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. ns, not significant