Fig. 6

The impairment of AhR activation induces DCs to prime T cell proliferation. The GM-CSF/IL-4-differentiated DCs were treated with or without 100 nM FICZ and then exposed with LPS to induce maturation of DCs for the next 24 h. The conditioned DCs were co-cultivated with autologous CFSE-labeled CD4⁺ T cells for 72 h (1:10 ratio of DC: CD4⁺ T), and T cell proliferation was determined by flow cytometry. The representative plots are shown in the figure