Fig. 5

CYPA and S100A4 induce p52 via STAT3 signaling. a Immunoblot (IB) using WI-38 cells incubated in regular media (RM) or conditioned media (CM) for 24 h, probed with the indicated antibodies. P-STAT3 (phospho-STAT3). b IB using lysate from WI-38 cells harvested on Day 0 and 7 following prolonged culture, probed with indicated antibodies. c IB using lysate from WI-38 cells at D0 and D7 treated with or without WP1066 (WP, 5 µM). d Quantification of β-gal-positive WI-38 cells on Day 7 following prolonged culture ± WP1066 (5 µM). Data represent mean value of three biological samples ± SEM. *P < 0.05 (two-tailed t test). e IB using lysate from WI-38 cells transfected with si-STAT3 or si-control (CTL) and incubated with either RM or CM for 24 h. Inset: IB of same cells with indicated antibody following transfection with siRNA. f IB using lysate from WI-38 cells incubated in recombinant CYPA or S100A4 for 24 h with or without WP1066 (5 µM). Blots are representative of at least two biologically independent experiments. Analysis of fold-change normalized to control lane shown below IB where indicated