Fig. 3

Conditioned media promotes p52 nuclear translocation. a Quantitative analysis (left) of β-gal-positive MEFs at indicated day following culturing with or without media change. Immunoblot (IB) (right) of same MEFs on Day 1 and 7 probed with anti-p52 and anti-Actin. b Quantification of β-gal-positive MEFs (left) on Day 3 following incubation with regular media (RM) or conditioned media (CM). Growth curves (right) of MEFs incubated in RM or CM passage 3 set as time 0. c IB using lysate from MEFs incubated in RM or CM for the specified time (hrs), probed with indicated antibodies. d IB using nuclear extract from MEFs incubated in RM or CM for 24 h. e qPCR analysis of mRNA from MEFs on Day 0 and 3 following incubation in RM or CM. Data show mean value of biological triplicate samples relative to Gapdh, ± SD normalized to Day 0. f IB with anti-p52 using lysate from WI-38 cells exposed to either RM or CM for 24 h. All data except qPCR represent mean value of three biological samples, ± SEM. *P < 0.05, **P < 0.01 (two-tailed t test). Blots are representative of at least two biologically independent experiments. Analysis of fold-change normalized to control lane shown below IB where indicated